Technical note: Development of DNA quantitation and STR typing systems for Panthera tigris species determination and individual identification in forensic casework

Authors

  • Daniel Vaněk Forensic DNA Service, Janovského 18, 170 00 Prague 7, Czech Republic; Institute of Legal Medicine, Bulovka Hospital, Prague, Czech Republic; 2nd Faculty of Medicine, Charles University in Prague, Czech Republic; Institute for Environmental Studies, Charles University in Prague, Czech Republic
  • Edvard Ehler Forensic DNA Service, Janovského 18, 170 00 Prague 7, Czech Republic; Department of Biology and Environmental Studies, Faculty of Education, Charles University in Prague, Czech Republic
  • Lenka Vaňková Forensic DNA Service, Janovského 18, 170 00 Prague 7, Czech Republic

DOI:

https://doi.org/10.14712/23361964.2021.13

Keywords:

CITES organism, DNA quantitation, forensic genetics, STR multiplex

Abstract

The aim of this technical note is to provide an overview of methodical approaches used to develop molecular systems for species determination/DNA quantification called Ptig Qplex and individual identification called Ptig STRplex of Panthera tigris samples. Both systems will help to combat the illegal trade of endangered species and create a worldwide shared database of DNA profiles.

Published

2021-12-14

Issue

Section

Articles